SARS-CoV-2 Shenanigans - A Walkthrough Analysis

submitted by raven9 from self.Coronavirus

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[–]infocom6502 1 insightful - 1 fun1 insightful - 0 fun2 insightful - 1 fun -  (3 children)

Thanks Raven.

All I can do is scratch my head at this point and hope some of this absorbs.

1246 GYIPE APRDGQAYVR L KDGEWVLLST FLGhhhhhhh

I did bing that difference (end portion of the 'Seattle' 6VXX_A Spike) and curiously the search engine points to a patent that has a vaguely similar sequence in it:

"The codons of CHA5 were optimized for expression by using human codons. As shown in Table 1, the original viral protease cleavage site PQRERRRKKRG (SEQ ID NO: 1) was mutated to PQRERG (SEQ ID NO: 2) in order to prevent proteins from the enzymatic cleavage to form HA1 and HA2. The transmembrane region (residues: 533-555) was replaced with the additional residues"

(SEQ ID NO: 3) LVPRGSPGSGYIPEAPRDGQAYVR KDGEWVLLSTFLGHHHHHH

"at the C terminus of the HA construct, where the thrombin cleavage site is in italics, the bacteriophage T4 fibritin foldon trimerization sequence is underlined" (GYIPEAPRDGQAYVRKDGEWVLLSTFLG), "and the His-tag is in bold (Stevens J, et al. (2006) Science 312:404-410)".

Thus is seems that the Seattle _A sequence has added the "bacteriophage T4 fibritin foldon trimerization sequence".

In summary, it seems that both the start and the tail of the Seattle strain has mystery additions, which is a total mystery to me at this point.

[–]raven9[S] 2 insightful - 1 fun2 insightful - 0 fun3 insightful - 1 fun -  (2 children)

I actually reworked this post on reddit partly because I completely messed up the sequence alignments (Late night posting lol) I have just now editted this one here with my fixed version and added more info. Thanks for looking at it and searching for more info and if you look since I fixed the sequence alignment you can clearly see the start and tail differences.

Please also look where I also added more info towards the end of the post because I looked at some other results of the blast search and found that identical sequence from gp41 was also in the spike protein from MERS!

If you look at the MERS genome link notice there is a link to a corresponding pubmed article from early 2019 https://pubmed.ncbi.nlm.nih.gov/30712865/

[–]infocom6502 1 insightful - 1 fun1 insightful - 0 fun2 insightful - 1 fun -  (1 child)

Thanks, I'll definitely look at it again soon.

I've suspected MERS as artificial for a long time and this looks like it's adding fuel to the fire:

other results of the blast search and found that identical sequence from gp41 was also in the spike protein from MERS!

[–]infocom6502 1 insightful - 1 fun1 insightful - 0 fun2 insightful - 1 fun -  (0 children)

the bacteriophage T4 fibritin foldon trimerization sequence is [....] GYIPEAPRDGQAYVRKDGEWVLLSTFLG

As far as the T4 fibritin foldon trimerization sequence, it seems now that MERS, HIV, and the Seattle modification of SARS2 all share this sequence with the T4 bacteriophage. Would be pretty mindblowing unless these were modified versions of the MERS, SARS2, and HIV.

HIV-1 -----gyipeap rdgqayvrkd gewvllstfl gssgneqel.......

SARS2 ggggsgyipeap rdgqayvrkd gewvllstfl ghhhhhhhh

MERS. ggggsgyipeap rdgqayvrkd gewvllstfl ghhhhhhhh