I have my own thoughts, but don't want to share them just yet to avoid biasing people.
EDIT: Since enough people have responded, my take is that the "Karen" meme is ultimately negative and feels very forced. Maybe it started organically, but since then it has been twisted for sinister political ends.
Here is the original KnowYourMeme article about the "Karen" meme. Note that there are zero political references. The gist of it is that "Karen" was originally just a joke, a meme character who was always asking to see the manager. She was never depicted as evil, just an annoying fictional trope similar to the hyperactive "Kyle" character.
Compare this with the current Wikipedia article, which by contrast is absolutely insane. It's clear that "Karen" just being a meme wasn't enough. It had to be repurposed, racialized, and used as a tool (and slur) against women who question forced rules; anything from mandatory mask-wearing policies to so-called "progressive" politics.
Originally, "Karen" was just a meme. After it was repurposed by political agents, now there are actual laws dedicated to identifying and punishing "Karens". It's nuts.